Info

Info

Pack HPLC Columns Yourself

Nowadays HPLC is one of the most effective chromatography methods for substance purification. Often the quality of the HPLC column and its costs are critical factors for the successful manufacturing and marketing of a product. Therefore, the possibility of packing preparative HPLC columns yourself in your own column packing station is a very cost-saving alternative to ready packed columns.


Key Features

  • Fast and easy packing even of very large HPLC columns.
  • No dead volume in the HPLC column by using dynamic axial compression.
  • High efficiency and long life span of the HPLC column due to best quality bed.
  • Cost savings through unpacking, cleaning of the column material and repacking.
  • No downtimes due to long delivery times for ready packed HPLC columns.
  • High flexibility for reacting quickly to new separation tasks.
  • Reduction of solvent consumption is possible through flexible length of the column bed.

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Packing Process

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No matter whether you are a well-trained technical user, or have no experience in packing HPLC columns – you can pack preparative HPLC columns in best quality in our column packing station within a few minutes yourself.

For the production of high quality HPLC columns a special packaging technique must be applied to compress the column material in the solid column tube made of stainless steel thus forming a homogeneous column bed. Only then the self-packed preparative column can be used for high pressure separation processes and provide distinct peaks after passing the HPLC detector.

For packing, the column material first is mixed in a container with a suitable solvent to an emulsion, referred to as slurry. The slurry must be stirred well, because only then a homogeneous column bed can be formed during subsequent compression in the column tube. The slurry is immediately filled into the column tube preventing it from settling down in the mixing vessel.

Now, the column piston is put into the column tube. During subsequent compression of the slurry the excess solvent escapes through the column outlet. Shortly afterwards, the specified packing pressure is reached and the pressing process is completed. The packing pressure must be significantly above the following operating pressure in the HPLC run.

The optimally designed distribution plate and the frit at the movable piston, guarantee a uniform distribution of the substance over the entire column cross-section resulting in optimal peaks.

Packing Process

For HPLC column usage with dynamic axial compression (DAC) the column remains in the column packing station and the column bed is automatically kept under pressure by the column piston. This prevents dead volume at the column inlet thus prolonging the life span of the preparative separation column massively whilst reducing ongoing operating costs at the same time.

SAC 25 mm ID

SAC 25 mm ID

DAC 100 mm ID

DAC 100 mm ID

DAC 300 mm ID

DAC 300 mm ID

DAC 800 mm ID

DAC 800 mm ID


A column packing station operated with static axial compression (SAC) makes manual repressing after prolonged use necessary to obtain a homogeneous column bed again. The HPLC column may be removed from both the DAC and the SAC column packing station e.g. for using the column separately. This way many columns can be prepared with a single packing station.

As previously mentioned, different column bed lengths can be packed with the column packing station which can contribute to cost savings for solvent. As an option temperature control over the full bed length is possible by a thermal jacket.

For HPLC columns with a diameter > 10 cm or in hygiene zones a slurry tank is used for the large amounts of column material and solvent. In the slurry tank, the slurry is mixed and pumped into the column tube located in the column packing station. This is, especially in the case of a large column volume, considerably easier for the user and can also be used in Ex zones.

Our column packing stations are distinguished by easy usability, the possibility of fast HPLC column packing and good reproducibility of the column bed. They combine highest quality with an extremely good price, saving your time and money. The range of services is rounded off by the possibility for using these column packing stations for the upscaling thus rendering the entire purification scale from milligram to kilogram possible.

Slurry Tank

Slurry Tank