Systems

SCPC Systems for Liquid-Liquid Extraction

The SCPC systems are a combination of HPLC with the SCPC as separation column for the purification of active ingredients by liquid-liquid extraction or distribution chromatography. They are ideally suited for laboratory, pilot plant and production.


Liquid-liquid extraction is also called liquid-liquid chromatography or distribution chromatography. The method belongs to the product range of column chromatography despite its column material free technology. Applications meet areas of e.g. pharmacognosy, pharmaceutical biology, organic chemistry, peptide chemistry, fermentations and soil ecology.

For liquid-liquid extraction the SCPC is attached to HPLC as a rotary separation column instead of a HPLC column with a solid stationary phase. Inside the SCPC a rotor with many round stacked metal plates is located. Inside the rotor more than a thousand chambers are interconnected in series.

SCPC 1000

SCPC

SCPC Rotor

Rotor

SCPC Rotor Chambers

Rotor Chambers

SCPC Distribution in the Chambers

Distribution in the Chambers

By means of the HPLC pump, the stationary liquid phase in the rotor is penetrated by a mobile liquid phase. The rotor is accelerated to about 2000 U / min, so that the centrifugal force causes the separation of the two liquid phases in the individual chambers. When the liquid mobile phase reaches equilibrium with the liquid stationary phase, the rotor is ready for sample injection.

The separation process of the liquid-liquid extraction takes place within the two immiscible phases whereby the components of the sample are distributed within the two liquid phases according to their distribution coefficients. The components move through the chambers at different rates to the rotor exit where they are fractionated. The combination of HPLC with the SCPC for liquid-liquid extraction allows separations of samples of various matrices in the analytical, preparative, and production scale.

The SCPC systems are schematically identical regardless of their size and throughput. The central unit of each system is the SCPC. It virtually resembles the separation column surrounded by peripheral devices, such as HPLC pump, sample injector, UV-VIS detector, fraction collector and computer with HPLC software for control and chromatogram evaluation.

Example 1: SCPC System for Laboratory

Liquid-Liquid Extraction System
Flow Sheet Liquid-Liquid Extraction Lab


Example 2: SCPC System for Pilot Plant and Production


HPLC Skid 6L ATEX

HPLC Skid 6L ATEX


SCPC 5000 ATEX

SCPC 5000 ATEX

Flow Sheet Liquid-Liquid Extraction Production


Key Features HPLC with SCPC

  • Injetion of varying sample volumes, low and high, at the same chromatographic conditions
  • Super-fast chromatography runs without interfering matrix effects
  • Cost-saving as no solid phase and only solvents necessary
  • Less solvent consumption compared to HPLC with solid phase column
  • Direct sample injection of e.g. crude extracts, without sample pretreatment
  • Quick and loss-free separation of crude extracts
  • Upscaling from milligram to kilogram scale
  • One hundred percent recovery of the ingredients
  • GMP, FDA and ATEX optional
  • Without irreversible adsorption
  • Ideal for samples of all polarities
  • Pressure stable up to 100 bar

Compared to solid-liquid chromatography, liquid-liquid extraction is trouble-less, simple and inexpensive, especially when crude extracts of different matrices have to be purified. The method does not directly compete with the solid-liquid HPLC though, but rather accelerates the entire chromatographic process to the pure product in combination with the latter. Further benefits of the liquid-liquid extraction using SCPC lie in the great savings of time and money, placing it centre stage as an innovative technology in general in contrast to the classical LC technologies in particular.


Some Versions of SCPC

SCPC with 100 mL Rotor Volume

100 mL Rotor Volume

SCPC with 250 mL Rotor Volume

250 mL Rotor Volume

SCPC with 1000 mL Rotor Volume

1000 mL Rotor Volume

SCPC with 5000 mL Rotor Volume

5000 mL Rotor Volume

There also are other versions of the SCPC. Just feel free to contact us, we will be very happy to answer your email or phone call.

Perhaps the following performance comparisons between LC / HPLC with solid phase column and LC / HPLC with SCPC are interesting for you.


Liquid-Liquid Extraction

Performance Comparison Solid Phase Column vs. SCPC

Example 1: Production of a Plant-Derived Active Ingredient

Basic Costs:
Laboratory costs and 1 employee 2,400€ / day, 220 working days, plant depreciation 6 years
Solvents per liter: ACN 32€, ethylacetat 26€, H2O 13€, heptane 36€, MeOH 14 €, BuOH 22€

. LC with Solid Phase Column LC with SCPC
Column/Rotor Glass Column ID 10 cm H 200 cm Rotor 6 Liters
Sample volume 250 g crude extract, 44 runs p.a. = 11.000 g 250 g crude extract, 110 runs p.a.= 27.500 g
Solvent per run 1.500 Liters of Heptan per run 1:1 150 Liters Heptan/ACN
Separation material Silicagel 90.000 € per year none
Duration for 250 g crude extract 5 working days for 250 g crude extract 2 working days
Costs LC-System 10.000 € /6 1.666 € R-System 230.000 € /6 38.333 €
Heptan 66.000 Liters 2.376.000 € Heptan 8.250 Liters 297.000 €
Heptan Recycling - 1.900.800 € Heptan Recycling - 237.600 €
ACN 8.250 Liters 264.000 €
ACN Recycling - 211.200 €
Separation material 90.000 € Separation material 0 €
Costs per year 566.866 € Costs per year 150.533 €
Costs per day 2.577 € Costs per day 684 €
Lab and 1 EE per day 2.400 € Lab and 1 EE per day 2.400 €
Total 5 days each 4.977 € / run 24.885 € 2 days each 3.084 € / run 6.168 €
Costs/g 24.885 € / 250 g 100 € 6.168 € / 250 g 25 €
g/h 250 g / 5 days = 40 h 6,25 g 250 g / 2 days = 16 h 15,6 g


Example 2: Production of a Medical Dye

Basic Costs:
Laboratory costs and 1 employee 2,400€ / day, 220 working days, plant depreciation 6 years
Solvents per liter: ACN 32€, ethylacetat 26€, H2O 13€, heptane 36€, MeOH 14 €, BuOH 22€

. LC with Solid Phase Column LC with SCPC
Column/Rotor Glass Column ID 5 cm H 100 cm Rotor 1 Liter
Sample volume 5 g sample, 55 runs p.a. = 275 g 5 g sample, 110 runs p.a. = 550 g
Solvent per run 20 Liter MeOH per run 1,5 Liter BUOH/10L H2O
Separation material Silicagel 1.100 € per year none
Duration for 5 g sample 4 working days for 5 g sample 2 working days
Costs LC-System 5.000 € /6 833 € R-System 80.000 € /6 13.333 €
MeOH 1.100 Liters 15.400 € BUOH 165 Liters 3.630 €
H2O 1.100 Liters 14.300 €
Separation material 1.100 Separation material 0 €
Costs per year 17.333 € Costs per year 31.263 €
Costs per day 79 € Costs per day 142 €
Lab and 1 EE per day 2.400 € Lab and 1 EE per day 2.400 €
Total 4 days each 2.479 € / run 9.916 € 2 days each 2.542 € / run 5.084 €
Costs/g 9.916 € / 5 g 1.983 € 5.084 € / 5 g 1.017 €
g/h 5 g / 4 days = 32 h 0,16 g 5 g / 2 days = 16 h 0,31 g


Example 3: Production of Peptides

Basic Costs:
Laboratory costs and 1 employee 2,400€ / day, 220 working days, plant depreciation 6 years
Solvents per liter: ACN 32€, ethylacetat 26€, H2O 13€, heptane 36€, MeOH 14 €, BuOH 22€

. LC with Solid Phase Column LC with SCPC
Column/Rotor HPLC-Column ID 10 cm H 25 cm Rotor 6 Liters
Sample volume 60 g sample, 220 runs p.a. = 13.200 g 60 g sample, 880 runs p.a.= 52.800 g
Solvent per run 70:30 54 Liter ACN/H2O per run 1:1 9 Liter BUOH/H2O
Separation material Silicagel 6.000 € per year none
Duration for 60 g sample 8 hours for 60 g sample 2 hours
Costs HPLC-System 120.000 € /6 20.000 € R-System 230.000 € /6 38.333 €
ACN 8.316 Liters 266.112 € BUOH 3.960 Liters 87.120 €
BUOH Recycling - 69.696 €
H2O 3.564 Liters 46.332 € H2O 3.960 Liters 51.480 €
Separation material 6.000 € Separation material 0 €
Costs per year 338.444 € Costs per year 107.237 €
Costs per day 1.538 € Costs per day 488 €
Lab and 1 EE per day 2.400 € Lab and 1 EE per day 2.400 €
Total 1 day each 3.938 € / run 3.938 € 2 hours each 361 € / run 722 €
Costs/g 3.938 € / 60 g 66 € 722 € / 60 g 12 €
g/h 60 g / 1 day = 8 h 7,5 g 60 g / 2 h 30 g


Example 4: Product Purification after Organic Synthesis

Basic Costs:
Laboratory costs and 1 employee 2,400€ / day, 220 working days, plant depreciation 6 years
Solvents per liter: ACN 32€, ethylacetat 26€, H2O 13€, heptane 36€, MeOH 14 €, BuOH 22€

. LC with Solid Phase Column LC with SCPC
Column/Rotor HPLC-Column ID 10 cm H 25 cm Rotor 6 Liters
Sample volume 60 g sample, 220 runs p.a. = 13.200 g 60 g sample, 880 runs p.a.= 52.800 g
Solvent per run 90:10 per run 1:1
Separation material Silicagel 6.000 € per year none
Duration for 60 g sample 8 hours for 60 g sample 2 hours
Costs HPLC-System 120.000 € /6 20.000 € R-System 230.000 € /6 38.333 €
Heptan 12.474 Liters 449.064 € Heptan 2.200 Liters 79.200 €
Heptan Recycling - 359.251 € Heptan Recycling - 63.360 €
Ethylacetat 1.386 Liters 36.036 € Ethylacetat 2.200 Liters 57.200 €
Ethylacetat Recycling - 28.829 € Ethylacetat Recycling - 45.760 €
MeOH 2.200 Liters 30.800 €
MeOH Recycling - 24.640 €
H2O 2.200 Liters 28.600 €
Separation material 6.000 € Separation material 0 €
Costs per year 123.020 € Costs per year 100.373 €
Costs per day 599 € Costs per day 456 €
Lab and 1 EE per day 2.400 € Lab and 1 EE per day 2.400 €
Total 1 day each 2.959 € / run 2.959 € 2 hours each 357 € / run 714 €
Costs/g 2.959 € / 60 g 49 € 714 € / 60 g 12 €
g/h 60 g / 1 day = 8 h 7,5 g 60 g / 2 h 30 g